(RS)-Benzyl mandelate

L-mandelate dehydrogenase activity

Flavocytochrome b # from Saccharomyces cereŠisiae is an lactate dehydrogenase which exhibits only barely detectable activity levels towards another 2-hydroxyacid, -mandelate. Using protein engineering methods we have altered the active site of flavocytochrome b # and successfully introduced substantial mandelate dehydrogenase activity into the enzyme. Changes to Ala-198 and Leu-230 have signi...

Constitutivity of the mandelate enzymes in Acinetobacter calcoaceticus, N.C.I.B. 8250, and its effect on the synthesis of benzyl alcohol dehydrogenase.

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Metabolism of mandelate and related compounds by bacterium NCIB 8250.

Bacterium NCIB 8250 was grown on mandelate, benzyl alcohol, benzoyl-formate, benzaldehyde or benzoate and also on 2-hydroxy, 4-hydroxy, 3,4-dihydroxy and 4-hydroxy-3-methoxy derivatives of these compounds. Growth rates and yields of organism were measured for many of the sub-strates. The pathways of oxidation of mandelate and related compounds were investigated by the technique of simultaneous ...

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